Fluorescence spectroscopy for beginners

Lecturer: dr inż. Krzysztof Żamojć

Laboratory classes contents:

spectrofluorometer operation; basic definitions and laws related with fluorescence spectroscopy; registration of absorption, fluorescence excitation and emission spectra; determination of fluorescence quantum yields; the studies of the influence of solvent's polarity on the fluorescence emission spectra; quantitative determination of the selected fluorophores.