Publikacje i patenty | Wydział Chemii

Lista prac w czasopismach recenzowanych

2016

Zylicz-Stachula A, Zebrowska J, Czajkowska E, Wrese W, Sulecka E, Skowron PM: Engineering TaqII bifunctional endonuclease DNA recognition fidelity: the effect of a single amino acid substitution within the methyltransferase catalytic site. Molecular Biology Reports 2016, DOI 10.1007/s11033-016-3949-3.

Zebrowska J, Zołnierkiewicza O, Skowron MA, Zylicz-Stachula A, Jezewska-Frackowiak J, Skowron PM: A putative type IIS restriction endonuclease GeoICI from Geobacillus sp. – a robust, thermostable alternative to mezophilic prototype BbvI. Journal of Biosciences 2016, DOI 10.1007/s12038-016-9595-z.

2015

Skowron MA, 2, Zebrowska J, Wegrzyn G, Skowron PM: MmoSTI restriction endonuclease, isolated from Morganella morganii infecting a tropical moth, Actias selene, cleaving 5’-|CCNGG-3’ sequences. J Appl Genetics 2015, DOI 10.1007/s13353-015-0308-3

Krefft D, Zylicz-Stachula A, Mulkiewicz E, Papkov A, Jezewska-Frackowiak J, Skowron PM: Two-stage gene assembly/cloning of TthHB27I, a member of a TspDTI-subfamily of bifunctional restriction endonucleases. Journal of Biotechnology 2015, 194:67-80.

Jezewska-Frackowiak J, Lubys A, Vitkute J, Zakareviciene l, Zebrowska J, Krefft D, Skowron M, Agnieszka Zylicz-Stachula A, Skowron PM: A new prototype IIS/IIC/IIG endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus, recognizing 5'-TARCCA(N11/9)-3' sequences. Journal of Biotechnology 2015, 194:19-26.

2014

Zylicz-Stachula A, Polska K, Skowron PM, Rak J: Artificial Plasmid Labeled with 5-Bromo- 2’-deoxyuridine: A Universal Molecular System for Strand Break Detection. ChemBioChem 2014, 15:1409-12.

Zylicz-Stachula A, Zolnierkiewicz O, Sliwinska K, Jezewska-Frackowiak J, Skowron PM: Modified 'one amino acid-one codon' engineering of high GC content TaqII-coding gene from thermophilic Thermus aquaticus results in radical expression increase. Microbial Cell  Factories 2014, 13:7. DOI: 10.1186/1475-2859-13-7.

Zylicz-Stachula A, Jezewska-Frackowiak J, Skowron PM: Cofactor analogue-induced chemical reactivation of endonuclease activity in a DNA cleavage/methylation deficient TspGWI N473A variant in the NPPY motif. Molecular Biology Reports 2014, DOI:10.1007/s11033-014-3085-x.

2013

Orlikowska M, Szymańska A, Borek D, Otwinowski Z, Skowron PM, Jankowska E.: Structural characterization of V57D and V57P mutants of human cystatin C, an amyloidogenic protein. Acta Crystallogr D Biol Crystallogr. 2013, 69:577-586.

Skowron PM, Vitkute J, Ramanauskaite D, Mitkaite G, Jezewska-Frackowiak J, Zebrowska J, Zylicz-Stachula A, Lubys, A: Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus. BMC Molecular Biology, 2013, 14:17.

Zylicz-Stachula A, Zolnierkiewicz O, Jasiecki J, Skowron PM: A new genomic tool: ultra- frequently cleaving TaqII/sinefungin endonuclease with combined 2.9 bp recognition site, applied to the construction of horse DNA libraries. BMC Genomics, 2013, 14:3720.

2012

Żylicz-Stachula A, Żołnierkiewicz O, Lubys A, Ramanauskaite D, Mitkaite G, Bujnicki JM, Skowron PM: Related bifunctional restriction endonuclease-methyltransferase triplets: TspDTI, Tth111II/TthHB27I and TsoI with distinct specificities. BMC Molecular Biology, 2012, 13:13.

2011

Żylicz-Stachula A, Żołnierkiewicz O, Śliwińska K, Jeżewska-Frąckowiak J, Skowron PM: Bifunctional TaqII restriction endonuclease: redefining the prototype DNA recognition site and establishing the Fidelity Index for partial cleaving. BMC Biochemistry , 2011, 12:62.

Orlikowska M, Jankowska E, Borek D, Otwinowski Z, Skowron P, Szymanska A: Crystallization and preliminary X-ray diffraction analysis of V57 mutants of an amyloidogenic protein - human cystatin C. Acta Crystallographica Section F, 2011, F67:1608-1611.

Żylicz-Stachula A, Żołnierkiewicz O, Jeżewska-Frąckowiak J, Skowron PM: Chemically-induced affinity star restriction specificity: a novel TspGWI/sinefungin endonuclease with theoretical 3-bp cleavage frequency. BioTechniques, 2011, 50:397-406.

Sobolewski I, Polska K, Żylicz-Stachula A, Jeżewska-Frąckowiak J, Rak J, Skowron PM: Enzymatic synthesis of long double-stranded DNA labeled with the haloderivatives of nucleobases in a precisely pre-determined sequence. BMC Biochemistry, 2011, 12:24.

Michalska B, Sobolewski I, Polska K, Zielonka J, Żylicz-Stachula A, Skowron PM, Rak J: PCR synthesis of double stranded DNA labeled with 5-bromouridine. A step towards finding a bromonucleoside for clinical trials. Journal of Pharmaceutical and Biomedical Analysis, 2011, 56:671-677.

2010 i wcześniejsze

Polska K, Zielonka J, Chomicz L, Czerwicka M, Stepnowski P, Guzow K, Wiczk W, Smużyńska M, Kasprzykowski F, Żylicz-Stachula A, Skowron PM, Rak J: Unexpected photoproduct generated via the acetone- sensitized photolysis of 5-Bromo-2’-deoxyuridine in a water/isopropanol solution: experimental and computational studies. Journal of Physical Chemistry B, 2010, 114: 16902-16907.

Żylicz-Stachula A, Bujnicki JM, Skowron PM: Cloning and analysis of bifunctional DNA methyltransferase/nuclease TspGWI, the prototype of a Thermus sp. family. BMC Molecular Biology, 2009, 10:52.

Skowron PM, Majewski J, Żylicz-Stachula A, Rutkowska SR, Jaworowska I, Harasimowicz-Słowińska R: A new Thermus sp. class-IIS enzymes subfamily: isolation of a "twin" restriction endonuclease TspDTI, with a novel specificity 5`-ATGAA(N11/9)-3` related to TspGWI, TaqII and Tth111II. Nucleic Acid Research, 2003, 31:e74.

Żylicz-Stachula A, Harasimowicz-Słowińska R, Sobolewski I, Skowron PM: TspGWI, a thermophilic class-IIS restriction endonuclease from Thermus sp. recognizes novel asymmetric sequence 5`-ACGGA(N11/9)-3`. Nucleic Acid Research, 2002, 30:e33.

Rutkowska SM, Skowron PM: Rapid screening of elution conditions prior to immunopurification of proteins. BioTechniques, 1999, 27:929-932.

Kaczorowski T, Sęktas M, Skowron PM, Podhajska AJ: The FokI methyltransferase from Flavobacterium okeanokoites. Purification and characterization of the enzyme and its truncated derivatives. Molecular Biotechnology, 1999, 13:1-15.

Swaminathan N, McMaster K, Skowron PM, Mead DA: Thermal cycle labeling: zeptomole detection sensitivity and microgram probe amplification using CviJI* restriction-generated oligonucleotides. Analytical Biochemistry, 1998, 255:133-141.

Swaminathan N, Mead D, McMaster K, George D, Van Etten JL, Skowron PM: Molecular cloning of the three base restriction endonuclease R.CviJI from eukaryotic Chlorella virus IL-3A. Nucleic Acid Research, 1996, 24:2463-2469.

Skowron PM, Harasimowicz R, Rutkowska SM: GCN4 eucaryotic transcription factor/FokI endonuclease mediated `Achilles Heel Cleavage`: quantitative study of protein-DNA interaction. Gene, 1996, 170:1-8.

Lee JH, Skowron PM, Rutkowska SM, Hong SS, Kim SC: Sequential amplification of cloned DNA as tandem multimers using class-IIS restriction endonucleases. Genetic Analysis: Biomolecular Engineering, 1996, 13:139-145.

Kim SC, Skowron PM, Szybalski W: Structural requirements for FokI-DNA interaction and oligodeoxyribonucleotide-instructed cleavage. Journal of Molecular Biology, 1996, 258:638-649.

Rutkowska SM, Skowron PM, Bielawski K, Podhajska A: SacNI, a novel BanII isoschizomer from Streptomyces achromogenes recognizing 5`-GRGCY/C-3`. Gene, 1995, 157:319-320.

Rutkowska SM, Skowron PM, Podhajska A: Purification and characterization of the restriction endonuclease AvcI from Actinomyces cristalomycini. Gene, 1995, 157:317-318.

Tucholski J, Skowron PM, Podhajska A: MmeI, a class-IIS restriction endonuclease: purification and properties. Gene, 1995, 157:87-92.

Skowron PM, Swaminathan N, McMaster K, George D, Van Etten J, Mead D: Cloning and application of the two/three-base restriction endonuclease R.CviJI from IL-3A virus-infected Chlorella. Gene, 1995, 157:37-41.

Skowron PM, Kaczorowski T, Tucholski J, Podhajska A: Atypical DNA-binding properties of class-IIS restriction endonucleases: evidence for recognition of the cognate sequence by a FokI monomer. Gene, 1993, 125:1-10.

Fitzgerald MC, Skowron PM, Van Etten JL, Smith LM, Mead DA: Rapid shotgun cloning utilizing the two base recognition endonuclease CviJI. Nucleic Acid Research, 1992, 20:3753-3762.

Piechula S, Skowron PM, Piatyszek M, Podhajska A: Isolation and identification of two new Synechococcus-derived restriction endonucleases, SleI and SspAI, isoschizomers of EcoRII. Nucleic Acid Research, 1991, 19:2782.

Kaczorowski T, Skowron PM, Podhajska AJ: Purification and characterization of the FokI Restriction endonuclease. Gene, 1989, 80:209-216.

Bielawski K, Skowron PM, Kur J, Konopa G, Podhajska A, Taylor K: Construction of a DNA-polymerase I overproducing plasmid and isolation of the enzyme. Acta Biochimica Polonica, 1987, 34:29-34.

Patenty

 

Skowron P, Jasiecki J: Sposób uzyskiwania rekombinantowej, biologicznie aktywnej butyrylocholinoesterazy końskiej i jej pochodnych oraz ogólny sposób uzyskiwania biologicznie aktywnych cholinoesteraz i butyrylocholinoestaraz oraz ich pochodnych w mikroorganizmie Leichmania tarentolae. Urząd Patentowy RP (2016)

Skowron P, Zylicz-Stachula A, Zolnierkiewicz O, Skowron M, Janus L, Jezewska-Frackowiak J, Krefft D, Nidzworski D, Szemiako K, Maciejewska N, Nowak M, Szymanska A: A method of obtaining a polyepitopic protein as well as a DNA vector for embodying this method. International Bureau of the World Intellectual Property Organization (2015), WO2015162560 A1.

Skowron P, Jeżewska-Frąckowiak J, Seroczynska K, Banaszczyk J, Wozniak D, Mazur P, Krefft D,Tadeusz Ossowski T:Transformowana komórka Escherichia coli  oraz sposób oceny skuteczności preparatów lub technik myjących w zmywaniu powierzchni. Urząd Patentowy RP (2015), nr P.415436

Skowron P, Zylicz-Stachula A, Zolnierkiewicz O, Skowron M, Janus L, Jezewska-Frackowiak J, Szymanska A: Sposób uzyskiwania białka poliepitopowego oraz wektor DNA do realizacji tego sposobu. Urząd Patentowy RP (2014), PK/2483/RW, in processing

Mead D, Skowron, PM, Swaminathan N, Van Etten J: Dinucleotide restriction endonuclease preparations and methods of use. European Patent EP 0690870 A1 (1996)

Nickerson D, Mueller R, Skowron P, Swaminathan N, Piehl R. DNA encoding a thermostable DNA polymerase enzyme. WO Patent 1,996,014,417 (1996)

Mueller R, Skowron P, Swaminathan N, Piehl R. Biologically active fragments of Thermsu flavus DNA polymerase. WO Patent 1,996,014,405 (1996)

Mueller R, Skowron P, Swaminathan N, Piehl R. Biologically active fragments of Thermsu flavus DNA polymerase. WO Patent 1,996,014,405 (1996)

Mead D, Skowron, PM, Swaminathan N, Van Etten J: Dinucleotide restriction endonuclease preparations and methods of use. European Patent EP 0690870 A1 (1996)

Mead D, Swaminathan N, Van Etten J, Skowron PM: Recombinant CviJI restriction endonuclease. United States Patent Office no US005472872A, USA (1995)

Mead D, Swaminathan N, Van Etten J, Skowron PM: Recombinant CviJI restriction endonuclease. United States Patent Office no US005472872A, USA (1995)

Mead D, Swaminathan N, Van Etten J, Skowron PM: Dinucleotide restriction endonuclease preparations and methods of use. WO Patent 1,994,021,663 (1994)

Treść ostatnio zmodyfikowana przez: Joanna Jeżewska-Frąckowiak
Treść wprowadzona przez: Joanna Jeżewska-Frąckowiak
Ostatnia modyfikacja: 
środa, 26 października 2016 roku, 10:10