Principles and applications of fluorescence spectroscopy

Lecturer: dr inż. Krzysztof Żamojć

Lecture contents:

Absorption of the light; ground and excited states; types of electronic transitions; absorption, excitation, and emission spectra; photophysical processes in the excited state; fluorescence quenching; the studies of the mechanisms of reactions - qualitative and quantitative methods; instrumentation.

Laboratory classes contents:

Spectrofluorometer operation; basic definitions and laws related with fluorescence spectroscopy; the studies of the mechanisms of fluorescence quenching; determination of aggregation number, cmc and hydrophobicity of the surfactants' micelles with the use of steady-state fluorescence emission spectra; the studies of the influence of various factors on the fluorescence emission spectra; determination of the stoichiometry and association constants of complexes.